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bdhA [2018-03-08 12:04:59]
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bdhA [2018-03-08 12:04:59]

acetoine/ butanediol dehydrogenase
Locus
BSU06240
Isoelectric point
4.80
Molecular weight
37.19 kDa
Protein length
346 aa Sequence Blast
Gene length
1038 bp Sequence Blast
Function
overflow metabolism, fermentation
Product
acetoine/ butanediol dehydrogenase
Essential
no
Synonyms
ydjL
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      
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Categories containing this gene/protein

  • Metabolism → Carbon metabolism → Carbon core metabolism → Overflow metabolism
  • Groups of genes → Phosphoproteins → Phosphorylation on an Arg residue

    • Gene

    Coordinates
    677,911 → 678,951

    The protein

    Catalyzed reaction/ biological activity

  • formation of butanediol from acetoin PubMed

    • Modification

  • phosphorylated on Arg-13 PubMed

    • Structure

  • 4EJ6 (from Sinorhizobium meliloti, 33% identity)

    • Additional information

  • belongs to the 100 most abundant proteins PubMed

    • Expression and Regulation

    Operons

    Genes
    bdhA
    Description
    PubMed

    Regulatory mechanism

  • AbrB: repression, PubMed, in AbrB regulon
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    Biological materials

    Mutant

  • MGNA-C219 (ydjL::erm), available at the NBRP B. subtilis, Japan
  • BKE06240 (ΔbdhA::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATGGATTACCACTCCTATA, downstream forward: _UP4_TAATTTGAAACCAAAAAGAA
  • BKK06240 (ΔbdhA::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATGGATTACCACTCCTATA, downstream forward: _UP4_TAATTTGAAACCAAAAAGAA

    • Two-hybrid system

  • B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Jörg Stülke's lab

    • References

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